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Updated: Oct 18, 2025
12:23
Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
Published on: August 2, 2018
12.3K
Multifunctionality of Prostatic Acid Phosphatase in Prostate Cancer Pathogenesis.
Evgenia Alpert1, Armin Akhavan2, Arie Gruzman2
1Bioconformatics laboratory of the California Pacific Medical Center (CPMC) Research Institute, Ramat-Gan, California, United States.
Bioscience Reports
|October 4, 2021
Summary
A novel proteomic tool revealed three distinct forms of human prostatic acid phosphatase (PAcP). One form, PLPAcP, is linked to early prostate cancer, suggesting new therapeutic targets.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Human prostatic acid phosphatase (PAcP) is implicated in prostate cancer.
- Understanding PAcP's diverse roles requires advanced proteomic techniques.
Purpose of the Study:
- To investigate the role of PAcP in prostate cancer using signal sequence swapping.
- To identify and characterize different functional forms of PAcP.
Main Methods:
- Utilized signal sequence swapping, a proteomic tool to alter protein biogenesis pathways.
- Analyzed PAcP's distinct forms based on physiological function, subcellular location, and biochemical properties.
Main Results:
- Identified three PAcP forms with identical amino acid sequences but different properties.
- Discovered that one form, PLPAcP, correlates with early-stage prostate cancer in clinical specimens.
Conclusions:
- The signal sequence swapping technique effectively reveals distinct protein subsets.
- PLPAcP plays a role in prostate cancer pathogenesis.
- Findings suggest novel therapeutic strategies targeting PLPAcP.
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