Immune Infiltration Pattern Associated with Diagnosis and Development in benign Prostatic Hyperplasia

Area of Science:

• Urology
• Immunology
• Genomics

Background:

• Benign prostatic hyperplasia (BPH) significantly impacts quality of life.
• The underlying biological mechanisms of BPH development are not fully understood.

Purpose of the Study:

• Investigate the essential genomic and immunogenic features in Benign prostatic hyperplasia (BPH).
• Identify key regulators and develop an immunological gene signature for BPH.

Main Methods:

• Acquired transcriptome profiling and clinical data from GEO datasets for BPH and normal prostate samples.
• Utilized ESTIMATE and CIBERSORT for immunogenic feature analysis.
• Performed transcriptional and weighted gene co-expression network analysis (WGCNA).

Main Results:

• BPH samples showed higher immune scores with abundant M2 Macrophages and follicular T helper cells.
• Identified SOCS3, IL6, C3, IGF1, NOTCH1, and VCAN as key regulators of the BPH immunogenic phenotype.
• Developed and validated an immunological gene signature for BPH.

Conclusions:

• BPH samples exhibit a distinct immune infiltration pattern characterized by an immunological gene signature.
• This genomic-based assessment model highlights potential transcriptomic patterns in BPH development.