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Related Experiment Video

Updated: Oct 18, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array
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A Portable Digital Loop-Mediated Isothermal Amplification Platform Based on Microgel Array and Hand-Held Reader.

Lei Cao1,2, Xiaojin Guo2,3, Ping Mao4

  • 1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

ACS Sensors
|October 4, 2021
PubMed
Summary
This summary is machine-generated.

A new portable digital loop-mediated isothermal amplification (dLAMP) platform simplifies DNA quantification. This low-cost system integrates sample partitioning and digital readout, enabling point-of-care testing for diseases.

Keywords:
digital LAMPhand-held readermicroscale hydrogel arraynucleic acid testingsample partition

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Diagnostics

Background:

  • Digital polymerase chain reaction (dPCR) offers sensitive molecular diagnostics but requires complex sample preparation and expensive equipment.
  • Current dPCR platforms are not suitable for point-of-care testing (POCT) due to operational complexity and cost.
  • There is a need for simplified, cost-effective nucleic acid quantification methods for decentralized testing.

Purpose of the Study:

  • To develop a portable and user-friendly digital loop-mediated isothermal amplification (dLAMP) platform.
  • To integrate sample partitioning, amplification, and digital readout into a single, cost-effective system.
  • To demonstrate the platform's capability for absolute DNA quantification and mutation detection for POCT applications.

Main Methods:

  • Development of a dLAMP platform incorporating a microscale hydrogel (microgel) array chip for self-contained sample partition.
  • Integration of a miniaturized heater for loop-mediated isothermal amplification and a hand-held reader for digital readout.
  • Quantification of lambda DNA (λDNA) and detection of epidermal growth factor receptor (EGFR) L858R gene mutation in spiked plasma samples.

Main Results:

  • The dLAMP platform achieved absolute quantification of λDNA with a linear detection range of 2-1000 copies/μL and a detection limit of 1 copy/μL.
  • Accurate identification of the EGFR L858R gene mutation was demonstrated in a spiked plasma sample.
  • The platform successfully integrated sample partitioning, amplification, and digital readout, eliminating the need for auxiliary equipment and complex operations.

Conclusions:

  • The developed portable dLAMP platform offers a low-cost, facile, and user-friendly solution for absolute DNA quantification.
  • The integrated microgel array chip enables efficient sample partition without external equipment.
  • This dLAMP platform shows significant potential for point-of-care testing (POCT) of nucleic acids, including disease-related gene mutations.