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Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
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Biosynthetic Glycan Labeling.

Victoria M Marando1, Daria E Kim1, Phillip J Calabretta1,2

  • 1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

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Summary
This summary is machine-generated.

Researchers developed a novel method to label glycans using cellular enzymes. This new probe selectively labels arabinofuranose in bacteria, aiding in mycobacterial detection and research.

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Area of Science:

  • Carbohydrate Chemistry
  • Chemical Biology
  • Microbiology

Background:

  • Glycans are crucial biomolecules, but studying them in cells is challenging due to limited chemical tools.
  • Existing methods for biomacromolecules like proteins are not directly applicable to glycans due to their structural similarities.

Purpose of the Study:

  • To develop a generalizable strategy for chemoselective glycan modification.
  • To create a tool for probing the structure and function of glycans within cellular environments.

Main Methods:

  • Designed bioorthogonal probes acting as substrate surrogates for glycosyltransferases.
  • Synthesized and tested a d-arabinofuranose (d-Araf) probe for mycobacterial cell walls.
  • Utilized glycosyltransferase promiscuity for selective labeling.

Main Results:

  • Developed the first probe for selective labeling of arabinofuranose-containing glycans.
  • Demonstrated the probe's ability to reveal asymmetric d-Araf distribution during mycobacterial growth.
  • Showed potential for detecting mycobacteria in macrophages.

Conclusions:

  • This approach provides a new platform for creating chemoselective labeling agents for various monosaccharides.
  • The d-Araf probe enables new insights into mycobacterial cell wall composition and dynamics.
  • The developed method has potential applications in pathogen detection.