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Analysis of Fatty Acid Content and Composition in Microalgae
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Tertiary amine as an efficient CO2 switchable solvent for extracting lipids from hypersaline microalgae.

Susaimanickam Anto1, M Premalatha1, Thangavel Mathimani1

  • 1Department of Energy and Environment, National Institute of Technology, Tiruchirappalli, 620015, Tamil Nadu, India.

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Summary

This study explored green solvents like Dimethylbenzylamine (DMBA) for extracting lipids from microalgae for biodiesel. DMBA showed high lipid yields from Chlorella sp. NITT05 and Picochlorum sp. NITT04, indicating their potential for biofuel production.

Keywords:
BiodieselChlorella sp.Picochlorum sp.Switchable solventTertiary aminesWet lipid extraction

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Area of Science:

  • Biotechnology
  • Renewable Energy
  • Green Chemistry

Background:

  • Microalgae are a sustainable source for biofuels.
  • Efficient lipid extraction is crucial for cost-effective algal biorefineries.
  • Traditional methods often involve harsh chemicals and energy-intensive processes.

Purpose of the Study:

  • To investigate switchable tertiary amine solvents for green lipid extraction from wet microalgae.
  • To evaluate the efficiency of Dimethylbenzylamine (DMBA), Dimethylcyclohexylamine (DMCHA), and Diisopropylethylamine (DIPEA).
  • To determine the suitability of specific microalgae strains for biodiesel production.

Main Methods:

  • Lipid extraction from wet hypersaline microalgae using DMBA, DMCHA, and DIPEA.
  • Optimization of solvent-to-biomass ratio (1:1 v/v) and extraction time (1 hour).
  • Analysis of fatty acid methyl ester (FAME) profiles to assess biodiesel quality.

Main Results:

  • DMBA achieved high lipid yields: 41.9% for Chlorella sp. NITT05, 33.3% for Picochlorum sp. NITT04.
  • DMCHA showed moderate yields (up to 34.28%), while DIPEA was ineffective.
  • Recovered DMBA solvent maintained high extraction efficiency.
  • FAME analysis indicated suitability of Chlorella sp. NITT05 and Picochlorum sp. NITT04 for biodiesel.

Conclusions:

  • DMBA is a promising green solvent for efficient microalgal lipid extraction.
  • Chlorella sp. NITT05 and Picochlorum sp. NITT04 are ideal candidates for biodiesel production.
  • This approach offers a cost-effective and environmentally friendly alternative for algal biorefineries.