Related Experiment Video
Updated: Oct 18, 2025

A Simple Protocol for Mapping the Plant Root System Architecture Traits
Published on: February 10, 2023
Physiological and molecular responses to combinatorial iron and phosphate deficiencies in hexaploid wheat seedlings
Gazaldeep Kaur1, Vishnu Shukla2, Varsha Meena2
1National Agri-Food Biotechnology Institute, Department of Biotechnology, Sector 81, Knowledge City, Mohali 140306, Punjab, India; Department of Biotechnology, Panjab University, Punjab, India.
Abstract:
Iron (Fe) and phosphorus (P) are the essential mineral nutrients for plant growth and development. However, the molecular interaction of the Fe and P pathways in crops remained largely obscure. In this study, we provide a comprehensive physiological and molecular analysis of hexaploid wheat response to single (Fe, P) and its combinatorial deficiencies. Our data showed that inhibition of the primary root growth occurs in response to Fe deficiency; however, growth was rescued when combinatorial deficiencies occurred. Analysis of RNAseq revealed that distinct molecular rearrangements during combined deficiencies with predominance for genes related to metabolic pathways and secondary metabolite biosynthesis primarily include genes for UDP-glycosyltransferase, cytochrome-P450s, and glutathione metabolism. Interestingly, the Fe-responsive cis-regulatory elements in the roots in Fe stress conditions were enriched compared to the combined stress. Our metabolome data also revealed the accumulation of distinct metabolites such as amino-isobutyric acid, arabinonic acid, and aconitic acid in the combined stress environment. Overall, these results are essential in developing new strategies to improve the resilience of crops in limited nutrients.
More Related Videos
09:13Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements
Published on: July 13, 2016
06:28Author Spotlight: Unraveling Plant Responses to Abiotic Stresses Using the PlantScreen Robotic Platform
Published on: June 7, 2024
Related Concept Videos
Responses to Drought and Flooding
Responses to Salt Stress
Responses to Heat and Cold Stress
Key Elements for Plant Nutrition
Stringent Response in E. coli