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Programmable nano-reactors for stochastic sensing.

Wendong Jia1,2, Chengzhen Hu1,2, Yuqin Wang1,2

  • 1State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 210023, Nanjing, China.

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Programmable nano-reactors for stochastic sensing (PNRSS) enable direct observation of single molecule reactions. This technique enhances sensing resolution for diverse chemical analyses, including drug screening.

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Area of Science:

  • Biophysics
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Observing single molecule chemical reactions is challenging due to rapid bond dynamics.
  • Biological nanopores can serve as single-molecule reactors but engineering is complex.
  • Current limitations hinder the development of nanopore-based single-molecule reaction analysis.

Purpose of the Study:

  • To introduce a versatile strategy, "programmable nano-reactors for stochastic sensing" (PNRSS), for observing single molecule reactions.
  • To demonstrate PNRSS's capability in analyzing a wide range of chemical reactions.
  • To showcase PNRSS's potential in drug screening and pharmacokinetic studies.

Main Methods:

  • Engineering biological nanopores into programmable nano-reactors.
  • Utilizing stochastic sensing to detect molecular events within the nanopore.
  • Applying artificial intelligence algorithms to enhance sensing resolution.

Main Results:

  • PNRSS successfully observed direct single molecule reactions of various compounds, including hydrogen peroxide, metal ions, and nucleoside analogues.
  • The technique demonstrated refined sensing resolution, further improved by AI algorithms.
  • PNRSS distinguished Remdesivir from its active triphosphate form, indicating potential for drug screening.

Conclusions:

  • PNRSS offers a versatile and effective platform for direct observation of single molecule reactions.
  • The enhanced sensing resolution and AI integration expand the applicability of nanopore sensing.
  • PNRSS shows promise for applications in drug screening and pharmacokinetic analysis.