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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
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RNA binding protein RBMS3 is a common EMT effector that modulates triple-negative breast cancer progression via

C James Block1, Allison V Mitchell1, Ling Wu1,2

  • 1Barbara Ann Karmanos Cancer Institute, Department of Oncology, Wayne State University School of Medicine, 4100 John R, Detroit, MI, 48201, USA.

Oncogene
|October 5, 2021
PubMed

Insights

RNA Binding Motif Single Stranded Interacting Protein 3 (RBMS3) drives breast cancer progression and metastasis by promoting the epithelial-to-mesenchymal transition (EMT). Targeting RBMS3 may offer a new therapeutic strategy for triple-negative breast cancer (TNBC).

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Epithelial-to-mesenchymal transition (EMT) is a key driver of tumor progression in breast cancer.
  • The role of RNA Binding Motif Single Stranded Interacting Protein 3 (RBMS3) in EMT and breast cancer has not been fully elucidated.

Purpose of the Study:

  • To investigate the function of RBMS3 in breast cancer, particularly in triple-negative breast cancer (TNBC).
  • To determine if RBMS3 is a potential therapeutic target for TNBC.

Main Methods:

  • Expression profiling in breast cancer cell lines.
  • Functional assays in immortalized mammary epithelial cell lines and TNBC models.
  • Genome-wide mRNA stability analysis.
  • Interaction studies between RBMS3 and PRRX1 mRNA.

Main Results:

  • RBMS3 is upregulated by EMT transcription factors and correlates with mesenchymal gene expression.
  • RBMS3 induces EMT and maintains the mesenchymal phenotype, invasion, and migration in TNBC models.
  • Loss of RBMS3 impairs tumor progression and metastasis in vivo.
  • RBMS3 enhances the stability of target mRNAs, including PRRX1, a key EMT transcription factor.

Conclusions:

  • RBMS3 is a novel and common effector of EMT in breast cancer.
  • RBMS3 plays a critical role in maintaining the mesenchymal phenotype and promoting metastasis in TNBC.
  • RBMS3 represents a promising therapeutic target for TNBC treatment.

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