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Related Experiment Video

Updated: Oct 18, 2025

Author Spotlight: Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

Author Spotlight: Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

Published on: April 14, 2023

Digital Light Processing Based Bioprinting with Composable Gradients.

Mian Wang¹, Wanlu Li¹, Luis S Mille¹

¹Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.

Advanced Materials (Deerfield Beach, Fla.)

|October 5, 2021

Summary

This summary is machine-generated.

A novel Digital Light Processing (DLP) bioprinting system with microfluidics enables real-time, customizable gradient fabrication for complex engineered tissues, minimizing waste and enhancing functionality.

Keywords:

Digital Light Processing bioprinting gradient structures microfluidic mixers vat polymerization

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Last Updated: Oct 18, 2025

Author Spotlight: Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

Author Spotlight: Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

Published on: April 14, 2023

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

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A Gradient-generating Microfluidic Device for Cell Biology

A Gradient-generating Microfluidic Device for Cell Biology

Published on: August 30, 2007

Area of Science:

Biomaterials Engineering
Tissue Engineering
Microfluidics

Background:

Reconstructing complex tissue gradients is crucial for engineered tissue functionality.
Existing methods struggle to efficiently create necessary gradients in 3D constructs.

Purpose of the Study:

To develop a composable-gradient Digital Light Processing (DLP)-based bioprinting system.
To integrate a microfluidic mixer for real-time gradient generation of bioinks.

Main Methods:

A DLP bioprinting system integrated with a microfluidic mixer was developed.
Real-time generation of continual or discrete gradients by adjusting bioink flow ratios.
Minimization of bioink waste during gradient exchange.

Main Results:

Demonstrated fabrication of planar and 3D structures with tunable gradients.
Achieved control over material composition, cell density, growth factor concentration, hydrogel stiffness, and porosity.
Gradients were created in horizontal and/or vertical directions.

Conclusions:

The developed system enables composable fabrication of multifunctional gradients.
This bioprinting technology holds significant potential for diverse biomedical applications.
The system offers a time- and bioink-saving strategy for gradient fabrication.