Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

2.1K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
2.1K
Microtubule Instability02:17

Microtubule Instability

5.4K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.4K
Destabilization of Microtubules01:45

Destabilization of Microtubules

2.9K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.9K
Microtubule Formation01:23

Microtubule Formation

6.4K
Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation...
6.4K
Anaphase A and B01:39

Anaphase A and B

4.3K
Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
4.3K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.2K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of spatiotemporal dynamics and driving factors of Zhejiang important agricultural heritage systems.

Scientific reports·2026
Same author

Gradient-Driven Galvanic Effect Enables Self-Sustained Peroxymonosulfate Activation in a Stacked Flow Reactor.

Environmental science & technology·2026
Same author

Machine learning models decipher environmental pollutant-driven core genes in glioblastoma: Biomarkers for diagnosis, recurrence, and prognosis.

Journal of environmental sciences (China)·2026
Same author

Orientated reception-emission of electrons at Fe single atoms driving emerging contaminants decomposition via Fe-O/C d-pπ conjugated effect.

Journal of hazardous materials·2026
Same author

Dual-Substrate Synergistic Photocatalysis: Exogenous Reagent-Free Co-Removal of Phenol and Cr(VI) via Electron-Donor-Mediated Redox Coupling over Modified Carbon Nitride.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Microorganism-mediated synergistic degradation of antibiotics in wastewater via dual-electric centers.

Bioresource technology·2026

Related Experiment Video

Updated: Oct 18, 2025

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
10:25

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

Published on: August 13, 2016

11.3K

Spatiotemporal changes in microtubule dynamics during dendritic morphogenesis.

Chun Hu1, Pan Feng1, Meilan Chen2

  • 1Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, China, Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou, China.

Fly
|October 5, 2021
PubMed
Summary

The Ste20-like kinase Tao regulates dendritic branching by controlling microtubule (MT) number. Tao kinase activity correlates with MT dynamics and dendritic morphogenesis, suggesting it limits branching by modulating MT abundance.

Keywords:
Microtubuledendritic morphogenesisdrosophilaneuronal developmentpolymerizationspatiotemporal changestao kinase

More Related Videos

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
07:43

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

Published on: November 20, 2021

3.2K
Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
08:31

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

6.3K

Related Experiment Videos

Last Updated: Oct 18, 2025

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
10:25

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

Published on: August 13, 2016

11.3K
In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
07:43

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

Published on: November 20, 2021

3.2K
Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles
08:31

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

6.3K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Dendritic morphogenesis relies on dynamic microtubules (MTs) for cytoskeletal organization.
  • Previous models proposed MT polymerization drives dendritic branching in Drosophila da neurons.
  • The kinase Tao regulates dendritic branching by affecting MT number, independent of polarity or speed.

Purpose of the Study:

  • Investigate the correlation between MT dynamics and dendritic morphogenesis.
  • Examine developmental regulation of Tao kinase activity in relation to MT dynamics and branching.
  • Elucidate Tao kinase's role in coordinating MT dynamics and dendritic development.

Main Methods:

  • Studied C1da and C4da neurons in Drosophila larvae.
  • Analyzed spatiotemporal changes in MT dynamics (number, polarity, speed).
  • Assessed dendritic branching patterns and Tao kinase activity over larval development.

Main Results:

  • Spatiotemporal changes in dynamic MT number correlated with dendritic branching.
  • Dynamic MT number changes also correlated with Tao kinase activity.
  • MT polarity and polymerization speed did not show a similar correlation.

Conclusions:

  • Tao kinase limits dendritic branching by controlling the abundance of dynamic MTs.
  • Findings suggest Tao kinase activity is developmentally regulated to coordinate MT dynamics and morphogenesis.
  • Propose a novel model for MT dynamics regulation in dendritic development.