Self-sorted Compartmentalization by Simultaneous Use of Natural and Synthetic Amphiphiles

Exploiting the orthogonal molecular interactions of natural (phospholipids) and synthetic (mono-allyloxylated cucurbit[7]uril) amphiphiles to form their own vesicles, the formation of two different types of compartments in a self-sorted manner mimicking cellular compartments is demonstrated. Even after simultaneous extrusion of both vesicles through small pore membranes, which transformed them into smaller vesicles, both vesicles were not fused but still appeared as independent compartments in sucrose solution. The simultaneous use of natural and synthetic amphiphiles, forming independent compartments, holds great potential for in-depth investigation of self-sorted multi-compartments and their structures as prototype cells.