Solubilization and degradation of extracellular matrix by various metastatic cell lines derived from a rat...

Examination by use of WAG syngeneic female rats was made on 4 rat rhabdomyosarcoma sublines expressing different metastatic potentials for their abilities to degrade proteoglycans and glycoproteins of the extracellular matrix (ECM), deposited by corneal endothelial cells and metabolically labeled with [3H]glycosamine and [35S]sulfate. Of the label incorporated in ECM, 10-20% was released in the culture medium in 3 days by the cell lines studied. The proteoglycans, glycosaminoglycans, and glycoproteins released by the cells from ECM were separated and partially characterized. Most of the ECM glycoproteins were recovered in the region of 200,000 (200K) on gel chromatography. Glycopeptides of 10K-20K apparent molecular weight were minor components. The glycosaminoglycan chains of the proteoheparan sulfate were partially hydrolyzed to 20K fragments. Tumor cells increased also the solubilization of the nondegraded matrix macromolecules. About 5-8% of the radioactivity of the ECM remained associated with the tumor cells detached from the ECM. ECM degrading activity of the sublines did not correlate with their ability to colonize the lungs after iv injection but did correlate with their ability to metastasize to the lungs from the primary subcutaneous site.