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Selective Monovalent Galectin-8 Ligands Based on 3-Lactoylgalactoside.

Benedetta Girardi1,2, Martina Manna1, Sjors Van Klaveren1,3

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Researchers developed improved molecular probes targeting galectin-8, a protein implicated in cancer and inflammation. These new ligands show enhanced affinity and selectivity, offering valuable tools for studying galectin-8's function in various diseases.

Keywords:
carbohydratesfluorescence polarizationgalectin-8galectinsmolecular probes

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Area of Science:

  • Biochemistry
  • Medicinal Chemistry
  • Pharmacology

Background:

  • Galectin-8 is a potential therapeutic target for cancer, inflammation, and bone disorders.
  • Understanding galectin-8 function requires specific molecular probes.
  • Existing galectin-8 ligands need improved affinity and selectivity.

Purpose of the Study:

  • To enhance the affinity and target selectivity of a known galectin-8 ligand.
  • To develop novel molecular probes for galectin-8 research.
  • To investigate galectin-8's role in disease through advanced probes.

Main Methods:

  • Chemical modification of a previously identified galectin-8 ligand (3-O-[1-carboxyethyl]-β-d-galactopyranoside).
  • Introduction of modifications at positions 1 and 3 of the galactose moiety.
  • Affinity measurement using fluorescence polarization assays.

Main Results:

  • The most potent modified compound exhibited a dissociation constant (KD) of 12 μM.
  • The developed ligands demonstrated reasonable selectivity against other galectins.
  • Successful optimization of ligand properties for galectin-8 targeting.

Conclusions:

  • The modified galectin-8 ligand represents a promising lead compound.
  • These novel ligands can serve as effective molecular probes for galectin-8.
  • Further studies in cell-based and in vivo models are warranted to explore galectin-8's functions.