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Related Experiment Video

Updated: Oct 17, 2025

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
10:25

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Published on: December 21, 2019

19.1K

Multi-material digital light processing bioprinting of hydrogel-based microfluidic chips.

Anant Bhusal1, Elvan Dogan1, Hai-Anh Nguyen1

  • 1Department of Mechanical Engineering, Rowan University, Glassboro, NJ, 08028, United States of America.

Biofabrication
|October 6, 2021
PubMed
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This study presents a novel digital-light-processing bioprinter for creating hydrogel microfluidic chips. The developed bioink supports cell viability and tissue development, advancing organs-on-chips technology.

Area of Science:

  • Biotechnology
  • Materials Science
  • Microfluidics

Background:

  • Advancements in digital-light-processing (DLP) bioprinting and hydrogel engineering are crucial for organs-on-chips development.
  • Existing methods may lack the speed and multi-material capabilities for rapid prototyping.

Purpose of the Study:

  • To design and develop a multi-material, DLP-based bioprinter for rapid, one-step prototyping of hydrogel-based microfluidic chips.
  • To optimize a composite hydrogel bioink for enhanced mechanical properties and cell compatibility.

Main Methods:

  • A multi-material DLP bioprinter was engineered for fabricating microfluidic chips.
  • A composite bioink of poly-ethylene-glycol-diacrylate (PEGDA) and gelatin methacryloyl (GelMA) was optimized by varying bioprinting parameters.
Keywords:
digital-light-processinghydrogel modelsmicrofluidicsorgan‐on‐a‐chip

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  • Mechanical properties and microfluidic features were characterized.
  • Human tumor cells were encapsulated to assess bioactivity and cell-friendly environment.

    • Main Results:

    • A wide range of mechanical properties were achieved for microfluidic chips by adjusting PEGDA:GelMA ratios.
    • Dynamic flow experiments confirmed the functionality of microfluidic features.
    • Encapsulated cells demonstrated bioactivity and a favorable environment within the 3D bioprinted structures.
    • Selected bioinks proved effective for vascularized micro-tissue engineering.

    Conclusions:

    • The developed DLP bioprinting approach enables rapid prototyping of functional hydrogel microfluidic chips.
    • This technology facilitates the integration of micro-tissue models for organs-on-chips and high-throughput drug screening.