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Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
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Related Experiment Video

Updated: Oct 17, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Phase interrogation surface plasmon resonance hyperspectral imaging sensor for multi-channel high-throughput

Ruibiao Miyan, Xueliang Wang, Jie Zhou

    Optics Express
    |October 7, 2021
    PubMed
    Summary

    This study introduces a novel multi-channel phase interrogation surface plasmon resonance (SPR) imaging system for high-throughput detection. The new design overcomes the narrow dynamic range limitation of previous phase interrogation SPR methods.

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    Area of Science:

    • Optics and Photonics
    • Biomedical Engineering
    • Analytical Chemistry

    Background:

    • Phase interrogation surface plasmon resonance (SPR) imaging offers potential for high-throughput, multi-sample detection.
    • Current phase interrogation SPR systems face limitations due to narrow dynamic range and variations in sample refractive indices, hindering multi-sample analysis.
    • Existing methods often require mechanical components, increasing complexity and reducing reliability.

    Purpose of the Study:

    • To design and demonstrate a novel multi-channel phase interrogation SPR imaging sensing scheme.
    • To overcome the limitations of narrow dynamic range and improve multi-sample detection capabilities in SPR imaging.
    • To develop a system without mechanical moving parts for enhanced stability and throughput.

    Main Methods:

    • A multi-channel phase interrogation SPR imaging system was designed utilizing a common optical interference path for p- and s-polarized light.
    • A birefringence crystal was employed to introduce a fixed optical path difference, generating sinusoidal spectral interference fringes.
    • A time-division-multiplexing peak-finding algorithm and an iterative parameter scanning cross-correlation algorithm were used for resonance wavelength tracking and phase value calculation.

    Main Results:

    • The developed scheme successfully enabled multi-channel detection without mechanical moving components.
    • The system demonstrated the ability to track resonance wavelengths across multiple channels, effectively expanding the detection range.
    • High-sensitivity phase values, indicative of sample properties, were accurately calculated for each channel.

    Conclusions:

    • The proposed multi-channel phase interrogation SPR imaging scheme effectively addresses the dynamic range limitations of conventional methods.
    • This innovative approach facilitates robust and high-throughput multi-sample analysis in SPR imaging.
    • The system's design, free from mechanical parts, offers a more stable and reliable platform for biosensing applications.