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Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

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Related Experiment Video

Updated: Oct 17, 2025

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

Airy light-sheet Raman imaging.

N R Subedi, S Yaraghi, P S Jung

|October 7, 2021

Summary

This summary is machine-generated.

Digitally scanned Airy beams significantly boost Raman imaging throughput for faster, more uniform 3D microscopy. This spectrometer-less method offers high contrast and resolution with reduced light exposure.

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Area of Science:

Biophotonics
Microscopy
Spectroscopy

Background:

Light-sheet microscopy enhances speed and photostability for cellular imaging.
Current light-sheet Raman imaging uses Gaussian beams, limiting field-of-view uniformity and throughput.

Purpose of the Study:

To demonstrate a novel light-sheet Raman imaging approach using digitally scanned Airy beams.
To improve imaging throughput rates and field-of-view characteristics compared to conventional methods.

Main Methods:

Employed a digitally scanned Airy beam in a light-sheet Raman imaging setup.
Developed a spectrometer-less approach for rapid 3D imaging.
Integrated with fluorescence light-sheet and quantitative-phase imaging.

Main Results:

Achieved over an order of magnitude increase in Raman imaging throughput.
Enabled 3D imaging of microparticles with high contrast and 1 µm axial resolution.
Demonstrated significantly lower irradiation density compared to coherent Raman imaging.

Conclusions:

Digitally scanned Airy beams offer a powerful solution for high-throughput, low-phototoxicity Raman microscopy.
The developed spectrometer-less system facilitates rapid multimodal imaging applications.
This technique advances optical sectioning for biological and material science investigations.