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Loading equine oocytes with cryoprotective agents captured with a finite element method model.

Sercan Içli1,2, Meisam Soleimani3, Harriëtte Oldenhof2

  • 1Biostabilization Laboratory - Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, NIFE, Stadtfelddamm 34, 30625, Hannover, Germany.

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Summary

Cryopreservation uses cryoprotective agents (CPAs) to preserve equine oocytes. This study models CPA and water transport to understand osmotic damage during cryopreservation, improving artificial reproduction outcomes.

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Area of Science:

  • Veterinary Medicine
  • Reproductive Biology
  • Biophysics

Background:

  • Cryopreservation enables long-term storage of equine oocytes for assisted reproduction.
  • Cryoprotective agents (CPAs) are essential for preventing freezing injury but can cause osmotic damage.
  • Oocyte response to CPAs depends on membrane permeability to water and CPAs.

Purpose of the Study:

  • To simulate equine oocyte volume changes and intracellular CPA concentrations during CPA exposure.
  • To develop a finite element method (FEM) model for spatial analysis of CPA permeation.
  • To enhance understanding of cryopreservation mechanisms through computational modeling.

Main Methods:

  • Developed a mathematical mass transport model for water and CPA permeation across the oocyte membrane.
  • Used analytical simulations to predict oocyte volume and CPA concentration.
  • Created a phenomenological FEM continuum model incorporating spatial information.

Main Results:

  • Simulated oocyte volume responses and intracellular CPA concentrations.
  • Depicted spatial CPA concentration differences within the oocyte during permeation.
  • Captured associated changes in oocyte deformation and hydrostatic pressure.

Conclusions:

  • FEM simulations provide valuable insights into CPA loading mechanisms in oocytes.
  • Understanding spatial CPA distribution is crucial for optimizing cryopreservation protocols.
  • This modeling approach can improve cryopreservation success rates in equine reproduction.