Related Experiment Video

Updated: Oct 17, 2025

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

4.7K

Bacterial Outer Membrane Vesicles as Antibiotic Delivery Vehicles

Shannon M Collins1, Angela C Brown1

  • 1Department of Chemical and Biomolecular Engineering, Lehigh University, Bethlehem, PA, United States.

Frontiers in Immunology
|October 7, 2021
PubMed

Insights

Bacterial outer membrane vesicles (OMVs) show promise as antibiotic delivery vehicles. These natural nanoparticles can overcome delivery challenges, aiding the fight against antibiotic resistance.

Area of Science:

  • Microbiology
  • Nanotechnology
  • Drug Delivery

Background:

  • Gram-negative bacteria release outer membrane vesicles (OMVs).
  • OMVs deliver biomolecules like toxins and virulence factors, impacting pathogenesis.
  • OMVs mediate bacterial communication (quorum sensing, gene transfer).

Purpose of the Study:

  • To review the use of OMVs as antibiotic delivery vehicles.
  • To discuss advantages, applications, and challenges of OMV-based antibiotic delivery.
  • To highlight the potential of OMVs in combating antibiotic resistance.

Main Methods:

  • Review of existing literature on OMVs and antibiotic delivery.
  • Analysis of natural and engineered OMV applications for antibiotics.
  • Evaluation of OMV properties for drug transport across bacterial envelopes.

Main Results:

  • OMVs can transport biomolecules across the Gram-negative cell envelope.
  • OMVs offer a strategy to overcome antibiotic transport limitations.
  • Both natural and engineered OMVs are being explored for antibiotic delivery.

Conclusions:

  • OMVs are versatile nanocarriers with significant potential for antibiotic delivery.
  • The use of OMVs is a promising approach to address antibiotic resistance.
  • Further research into biotechnological challenges is needed for OMV applications.
Keywords:
Gram-negative bacteriaantibiotic resistanceantibioticsdrug deliveryouter membrane vesicles

More Related Videos

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.8K
Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria06:38

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

1.2K

Related Concept Videos

Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
238
COP Coated Vesicles00:59

COP Coated Vesicles

Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
13.2K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.6K
Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...
496
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
155
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
180