m⁶A Methylation Modification Patterns and Tumor Microenvironment Infiltration Characterization in Pancreatic Cancer

Recent studies have shown that RNA N⁶-methyladenosine (m⁶A) modification plays an important part in tumorigenesis and immune-related biological processes. However, the comprehensive landscape of immune cell infiltration characteristics in the tumor microenvironment (TME) mediated by m⁶A methylation modification in pancreatic cancer has not yet been elucidated. Based on consensus clustering algorithm, we identified two m⁶A modification subtypes and then determined two m⁶A-related gene subtypes among 434 pancreatic cancer samples. The TME characteristics of the identified gene subtypes were highly consistent with the immune-hot phenotype and the immune-cold phenotype respectively. According to the m⁶A score extracted from the m⁶A-related signature genes, patients can be divided into high and low m⁶A score groups. The low score group displayed a better prognosis and relatively strong immune infiltration. Further analysis showed that low m⁶A score correlated with lower tumor mutation burden and PD-L1 expression, and indicated a better response to immunotherapy. In general, m⁶A methylation modification is closely related to the diversity and complexity of immune infiltration in TME. Evaluating the m⁶A modification pattern and immune infiltration characteristics of individual tumors can help deepen our understanding of the tumor microenvironment landscape and promote a more effective clinical practice of immunotherapy.