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Paper-based aptamer-antibody biosensor for gluten detection in a deep eutectic solvent (DES).

Rossella Svigelj1, Nicolò Dossi2, Cristian Grazioli2

  • 1Department of Agrifood, Environmental and Animal Science, University of Udine, via Cotonificio 108, 33100, Udine, Italy. svigelj.rossella@spes.uniud.it.

Analytical and Bioanalytical Chemistry
|October 7, 2021
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Summary

A novel paper-based biosensor detects gluten in food using aptamers and antibodies. This low-cost electrochemical sensor, utilizing a deep eutectic solvent, achieves high sensitivity for celiac disease patients.

Keywords:
AptamersDeep eutectic solventsElectrochemical detectionGlutenPaper-based biosensor

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Area of Science:

  • Analytical Chemistry
  • Biosensors
  • Materials Science

Background:

  • Paper is a cost-effective material for developing various sensors, including those for medical diagnostics.
  • There's a growing demand for accessible, low-cost analytical devices for both consumers and laboratory use.
  • Existing gluten detection methods may lack the sensitivity required for highly gluten-sensitive individuals.

Purpose of the Study:

  • To develop a novel paper-based electrochemical sensing platform for gluten detection.
  • To integrate aptamers and antibodies with a deep eutectic solvent (DES) for enhanced gluten analysis.
  • To create a sensitive and low-cost biosensor suitable for individuals with celiac disease.

Main Methods:

  • Modification of a paper disc with recognition molecules (aptamers and antibodies).
  • Utilizing a screen-printed carbon electrode (SPCE) for electrochemical detection.
  • Employing the deep eutectic solvent 'ethaline' for gluten extraction and analysis.

Main Results:

  • The developed biosensor successfully detects gluten in ethaline.
  • Achieved a low limit of detection (LOD) of 0.2 mg L-1 for gluten.
  • Demonstrated a dynamic range of 0.2–20 mg L-1 with an intra-assay coefficient of 10.69%.

Conclusions:

  • The paper-based electrochemical biosensor offers a sensitive method for gluten detection.
  • Ethaline serves as an effective extraction medium, enabling the detection of low gluten concentrations.
  • This technology is highly relevant for gluten-sensitive individuals needing to monitor gluten intake below legal limits.