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Published on: August 8, 2016
Influence of Xenon on Survival of Sperm of Common Frog Rana temporaria during Slow Freezing
S A Kaurova1, N E Shvirst2, N V Shishova2
1Institute of Cell Biophysics, Russian Academy of Sciences - Separated Subdivision of the Federal Research Center Pushchino Scientific Center of Biological Research, Russian Academy of Sciences, Pushchino, Russia. sakaurova@mail.ru.
Abstract:
We studied the effect of xenon on the survival rate of the spermatozoa of the common frog Rana temporaria during slow freezing with saturation of the suspension with xenon at a pressure of up to 1.2 bar. The cryoprotective properties of xenon were analyzed in comparison with nitrogen. No specific cryoprotective effect of xenon was revealed. Viability of spermatozoa pretreated with xenon at atmospheric pressure (0 bar) or under excess pressure of 0.6 bar and frozen in a cryoprotective medium with dimethylformamide, sucrose, and BSA did not differ significantly. The use of overpressure of xenon of 1.0 or 1.2 bar in the pretreatment and freezing process significantly impaired viability of the biomaterial.

