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A method for high-throughput image-based antifungal screening.

Rosalie Sabburg1, Aphrika Gregson2, Andrew S Urquhart3

  • 1Commonwealth Scientific and Industrial Research Organisation, St Lucia, Queensland, Australia.

Journal of Microbiological Methods
|October 7, 2021
PubMed
Summary

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Developing a rapid antifungal screening method is crucial for identifying new treatments. This study introduces a time-resolved, image-based assay for filamentous fungi, enabling quick and accurate susceptibility testing within hours.

Area of Science:

  • Mycology
  • Pharmaceutical Sciences
  • Biotechnology

Background:

  • Antifungal drug discovery is vital due to rising resistance.
  • Screening filamentous fungi presents significant technical hurdles.
  • Existing methods are often time-consuming and lack precision.

Purpose of the Study:

  • To develop and validate a novel, rapid antifungal screening assay.
  • To improve the efficiency and accuracy of antifungal susceptibility testing.
  • To facilitate the discovery of new antifungal compounds.

Main Methods:

  • Utilized a modified broth dilution protocol.
  • Incorporated time-resolved, image-based assessment of fungal growth.
  • Validated the method with diverse fungal species and compound classes.
Keywords:
AntifungalAspergillusFusariumRapidSusceptibilityVerticillium

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Main Results:

  • The assay accurately determined fungal susceptibility within hours.
  • Demonstrated efficacy with various fungi, especially spore-forming types.
  • Successfully accounted for inherent differences in fungal strain growth rates.

Conclusions:

  • The presented method offers a robust and efficient approach for antifungal screening.
  • This technique accelerates the identification of potential antifungal agents.
  • It overcomes key limitations of traditional antifungal assays.