Effects of leaf removal on hexose accumulation and the expression of sugar unloading-related genes in syrah grapes

Leaf removal (LR) around the cluster zone is a common practice for regulating grape quality. The purpose of this study was to assess the effects of cluster-zone leaf removal, applied at the pea-size stage of berry development, on berry soluble sugar, organic acid and phenolic compound, sugar unloading-related gene expression of Vitis. vinifera L. Syrah. Four different severities of leaf removal were applied: no leaf removal (Control), removing 2 leaves above the cluster (LR1), removing 4 leaves above the cluster (LR2), and removing 6 leaves above the cluster (LR3). The three leaf removal treatments (LR), especially removing 4 leaves (LR2), resulted in significantly higher reducing sugar, soluble sugar (glucose, fructose and sucrose), total anthocyanin and citric acid contents as compared to the control group during ripening for both vintages. At harvest, the LR treatments increased the transcript abundance of most sugar unloading-related genes. In addition, VvHT3, VvHT5, VvSUC11, VvSUC12, VvSS and VvcwINV were positively correlated with both reducing sugar contents and soluble sugar contents. Our results suggest that removing 4 leaves above the cluster is useful for improving the quality of Syrah (Vitis vinifera L.) grapes in cool climate regions with excessive leaves. These findings provide insights into the molecular basis of the relationship between leaf removing and hexose (glucose and fructose) accumulation in the grape berries.