Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

655
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
655
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

418
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
418
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

524
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
524
In-situ Hybridization02:31

In-situ Hybridization

9.7K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.7K
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

268
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
268
Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

704
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
704

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multicenter evaluation of BACT-Info. and an infection algorithm using Urine Flow Cytometry among clinically diagnosed UTI patients in Indonesia.

PloS one·2026
Same author

Immune Dysregulation and Community Health Factors in Maternal and Childhood Leprosy: A Partial Least Squares-Structural Equation Modelling Study.

Infection & chemotherapy·2026
Same author

Basic fibroblast growth factor preserves rabbit adipose-derived mesenchymal stromal cells in serum-free culture.

Journal of veterinary science·2026
Same author

Impact of atopic dermatitis on education and career choices: the Scars of Life international study.

The Journal of investigative dermatology·2026
Same author

The Influence of Different Anatomical Sites on the Characteristics and Differentiation Capacity of Rabbit Adipose-Derived Mesenchymal Stem Cells.

Veterinary medicine international·2026
Same author

Lessons learned from life-threatening respiratory and cardiac complications in diphtheria: A case report and literature review.

Turkish journal of emergency medicine·2026

Related Experiment Video

Updated: Oct 17, 2025

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
07:35

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Published on: June 7, 2017

9.9K

Characterization of SARS-CoV-2 East Java isolate, Indonesia.

Fedik Abdul Rantam1,2, Cita Rosita Sigit Prakoeswa3, Damayanti Tinduh4

  • 1Research Center for Vaccine Technology and Development, Institute of Tropical Disease, Airlangga University, Surabaya, East Java, 60132, Indonesia.

F1000Research
|October 8, 2021
PubMed
Summary

This study characterized SARS-CoV-2 isolates from East Java, Indonesia, identifying key mutations like D614G and specific lineages. The findings reveal insights into the virus

Keywords:
COVID-19SARS-CoV-2growth propertiesinfectious diseasevirus isolation

More Related Videos

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.3K
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.9K

Related Experiment Videos

Last Updated: Oct 17, 2025

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
07:35

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Published on: June 7, 2017

9.9K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.3K
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.9K

Area of Science:

  • Virology
  • Genomics
  • Immunology

Background:

  • East Java, Indonesia, became a COVID-19 epicenter with rising SARS-CoV-2 cases.
  • The COVID-19 pandemic presented a global multisectoral crisis.
  • Characterization of local SARS-CoV-2 isolates is crucial for understanding regional epidemiology.

Purpose of the Study:

  • To investigate and characterize local SARS-CoV-2 isolates from East Java, Indonesia.
  • To analyze the genetic makeup and mutations of the virus.
  • To assess the immunogenicity of the SARS-CoV-2 isolates.

Main Methods:

  • Virus isolation from nasopharyngeal swabs of COVID-19 positive patients using Vero E6 cell lines.
  • Confirmation of SARS-CoV-2 infection via RT-PCR, scanning electron microscopy, and transmission electron microscopy.
  • Whole genome sequencing using ARTIC protocol and characterization through Western blot analysis.

Main Results:

  • Three isolates (#6, #11, #35) showed good growth characteristics.
  • The D614G mutation was detected in all isolates; one isolate also had D215Y and E484D mutations.
  • Whole genome analysis placed isolates in clade 20A, with lineages B.1.6 and B.1.4.7 identified.

Conclusions:

  • Molecular characterization of SARS-CoV-2 from East Java revealed significant mutations.
  • The identified isolates demonstrated high immunogenicity.
  • Understanding regional viral characteristics and mutations is vital for public health strategies.