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Role of miR-490-3p in blocking bladder cancer growth through targeting the RNA-binding protein PCBP2
Cun-Ming Zhang1, Li-De Song2, Jun-Wei Wang1
1Department of Urinary Surgery, The Affiliated Wenling Hospital of Wenzhou Medical University, Taizhou, Zhejiang Province, China.
Abstract:
MiR-490-3p is regarded as a tumor suppressor in many cancers, but whether miR-490-3p is involved in the development of bladder cancer remains unknown. BALB/c nude mice (male, 15-20 g) were used to investigate the role of MiR-490-3p in bladder cancer. The relationship between miR-490-3p and PCBP2 involved in bladder cancer regulation were determined. Cell viability, proliferation, and cell cycle were estimated by cell counting kit-8 (CCK-8) assay, 5-bromo-2'-deoxyuridine (BrdU) detection, and flow cytometry analysis, respectively. In animal experiments, lentivirus was transfected into bladder cancer cells to overexpress miR-490-3p, which were then injected into mice and the change of tumor volume was assessed. Principal findings: The expression of MiR-490-3p was decreased in bladder cancer cells. Overexpression of miR-490-3p inhibited bladder cancer cell viability and proliferation. Moreover, overexpression of miR-490-3p caused cell cycle arrest in bladder cancer cells. The inhibitory effect of miR-490-3p on bladder cancer cells growth could be counteracted by enhancing PCBP2 expression. In vivo, bladder cancer growth in mice was blocked by miR-490-3p upregulation. MiR-490-3p suppressed bladder cancer growth and bladder cancer cell proliferation by down-regulating PCBP2 expression.
Insights
MicroRNA-490-3p (miR-490-3p) acts as a tumor suppressor in bladder cancer by inhibiting cell viability and proliferation. Upregulating miR-490-3p suppressed tumor growth in vivo by downregulating PCBP2 expression.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNA-490-3p (miR-490-3p) is recognized as a tumor suppressor in various cancers.
- Its specific role in bladder cancer development and progression is not yet fully elucidated.
Purpose of the Study:
- To investigate the function of miR-490-3p in bladder cancer.
- To determine the relationship between miR-490-3p and PCBP2 in bladder cancer regulation.
Main Methods:
- Cell viability, proliferation, and cell cycle were assessed using CCK-8, BrdU detection, and flow cytometry.
- In vivo studies involved overexpressing miR-490-3p in bladder cancer cells via lentivirus transfection in BALB/c nude mice.
- Tumor volume changes were monitored to evaluate the effect of miR-490-3p.
Main Results:
- miR-490-3p expression was found to be decreased in bladder cancer cells.
- Overexpression of miR-490-3p significantly inhibited bladder cancer cell viability, proliferation, and induced cell cycle arrest.
- The tumor suppressor role of miR-490-3p was linked to the downregulation of PCBP2 expression, which could counteract its inhibitory effects.
- In vivo experiments demonstrated that miR-490-3p upregulation effectively blocked bladder cancer growth in mice.
Conclusions:
- miR-490-3p functions as a tumor suppressor in bladder cancer.
- miR-490-3p inhibits bladder cancer cell growth and proliferation, partly by downregulating PCBP2 expression.
- Targeting miR-490-3p or its downstream effectors may offer therapeutic strategies for bladder cancer.
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