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Updated: Oct 17, 2025

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
Published on: November 29, 2014
Searching for methyllysine-binding aromatic cages
Kendra R Vann1, Yashavantha L Vishweshwaraiah2, Nikolay V Dokholyan2,3
1Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, U.S.A.
Abstract:
Methylation of lysine residues plays crucial roles in a wide variety of cell signaling processes. While the biological importance of recognition of methylated histones by reader domains in the cell nucleus is well established, the processes associated with methylation of non-histone proteins, particularly in the cytoplasm of the cell, are not well understood. Here, we describe a search for potential methyllysine readers using a rapid structural motif-mining algorithm Erebus, the PDB database, and knowledge of the methyllysine binding mechanisms.
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