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Updated: Oct 17, 2025
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
Near-infrared-based hematocrit prediction of dried blood spots: An in-depth evaluation
Lisa Delahaye1, Liesl Heughebaert1, Christoph Lühr2
1Laboratory of Toxicology, Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent, Belgium.
Background:
Dried blood spot (DBS) microsampling has gained interest in different clinical fields, owing to its many advantages compared to conventional blood sampling. However, whilst being applied for decades for screening purposes, some challenges, such as the hematocrit (Hct) effect, hinder further widespread use of DBS for quantitative purposes in clinical practice. Amongst the approaches that were developed to cope with this issue, is the Hct prediction of DBS using near-infrared (NIR) spectroscopy.
Methods:
Using left-over EDTA-anticoagulated patient samples, the accuracy and precision, stability, and robustness were assessed. Furthermore, applicability of the method on capillary DBS was evaluated via finger prick samples.
Results:
A maximal bias, respectively CV, of 0.012 L/L and 4.5% were obtained. The method was robust towards several aspects, including storage (except for storage at 60°C), measurement location, type of filter paper and spotted volume. Furthermore, the potential to predict the Hct of capillary DBS was demonstrated.
Conclusion:
A commercially available NIR set-up was extensively and successfully validated, allowing non-contact Hct prediction of DBS with excellent accuracy and precision. This allows to correct for the Hct-based bias observed in partial-punch DBS analysis and the set-up of blood-plasma conversion factors, increasing the application potential of patient-centric sampling.
