Enrichment of Clostridia enhances Geobacter population and electron harvesting in a complex electroactive biofilm

Current research on microbial fuel cell or microbial electrolysis cell dealt with finding new electroactive bacteria and understanding the mechanisms of electronic exchange. Complex consortia allowed to obtain better performances than pure cultures in part thanks to inter-species cooperation. However, the role of each bacterium in a complex biofilm in the electron harvest on an electrode remains unclear. Thus, we combined electrochemical monitoring of electron exchange and high throughput sequencing analysis in order to describe the bacterial composition and the electroactive performance of mangrove mud biofilms. In this study, secondary electroactive biofilms were formed on carbon electrodes from Desulfuromonas-dominated inoculum of pre-formed bioanodes. The performances and the Desulfuromonas-dominated profile were the same as those of primary bioanodes when the planktonic community was conserved. However, a Clostridium enrichment allowed to restore the performance in maximal current densities promoting an increase of Geobacter population, becoming the most dominant group among the Deltaproteobacteria, replacing Desulfuromonas. These results highlight a positive collaboration between Clostridium and Geobacter spp. helping a bacterial population to achieve with the depletion of their environment. Our study provides new insight into relationships between dominant electroactive bacteria and other bacteria species living in an organic matter-rich environment as mangrove sediments.