Rapid development and optimization of paper microfluidic designs using software automation

Paper microfluidic or lateral flow devices have found many applications, especially in medical diagnostics. Their low cost and ease of use makes them particularly valuable in resource-limited and point-of-care applications. However, the process of developing new paper microfluidic devices is slowed by the need to find optimal values for their various design parameters, which determine the overall size and fluid volume requirements of the device. Often, researchers must design and test several different versions of a device to find a combination of parameters that functions as intended. To accelerate the development of new paper microfluidics, we developed a software framework that automatically designs custom paper microfluidic devices for a given application. Once the user specifies the desired device parameters, the software generates printable image files of the resulting devices, ready to output to a conventional wax ink color printer and test. As a proof-of-concept, we used our software to automatically design 51 different paper microfluidic devices we needed to create a functional lateral flow assay that detects protein and glucose in urine. These designs took only a few seconds to generate and were used in 120 lab experiments we performed in 16 h in the lab. Thus, with the help of our software framework, we went from an idea to a functional device in just two work days. By accelerating device development and enabling researchers without microfluidics experience to create custom devices, our software can help spread paper microfluidic technology to important new application areas.