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Methods for Assessing the Effects of Xylosides on Angiogenesis.
Jie Shi Chua1, Geethu Muruganandam1, Yukio Saijoh2
1Departments of Medicinal Chemistry, Biology and Bioengineering, University of Utah, Salt Lake City, UT, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 9, 2021
Summary
Synthetic xylosides prime glycosaminoglycan assembly and show therapeutic potential. Researchers developed a method to screen xylosides for their ability to promote or inhibit angiogenesis, vital in many diseases.
Area of Science:
- Biochemistry
- Molecular Biology
- Drug Discovery
Background:
- Xylosides are synthetic molecules comprising a xylose unit and an aglycone.
- They function as primers for glycosaminoglycan (GAG) synthesis.
- GAG biosynthesis is crucial in various biological processes and diseases.
Purpose of the Study:
- To develop a general strategy for screening xylosides.
- To evaluate the capacity of xylosides to modulate angiogenesis.
- To explore xylosides as potential therapeutics targeting GAG biosynthesis.
Main Methods:
- Comprehensive screening of synthetic xylosides.
- Assessment of their effects on glycosaminoglycan biosynthesis.
- Evaluation of their pro-angiogenic or anti-angiogenic activities.
Main Results:
- Identified specific xylosides with selective priming and inhibitory activities on GAG biosynthesis.
- Demonstrated the ability of xylosides to promote or inhibit angiogenesis.
- Established a versatile screening approach for xyloside-based therapeutics.
Conclusions:
- Synthetic xylosides offer a promising avenue for therapeutic development.
- Targeting GAG biosynthesis with xylosides can modulate angiogenesis.
- The developed screening strategy enables the discovery of novel xyloside therapeutics for angiogenesis-related diseases.
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