Analysis of 3'-Phosphoadenosine 5'-Phosphosulfate Transporters: Transporter Activity Assay, Real-Time Reverse
Hideo Egawa1, Shoko Nishihara2,3
1Department of Bioinformatics, Graduate School of Engineering, Soka University, Hachioji, Tokyo, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|October 9, 2021
Abstract:
3'-Phosphoadenosine 5'-phosphosulfate transporters (PAPSTs) play an important role in transporting 3'-phosphoadenosine 5'-phosphosulfate (PAPS), the universal sulfuryl donor for sulfation, from the cytosol into the lumen of the Golgi apparatus. Here, we describe three methods for the analysis of PAPST; a transporter activity assay with yeast or mammalian cell fraction, real-time reverse transcription polymerase chain reaction on tissue samples, and immunohistochemistry on brain sections.
