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Microbial explanation to performance stratification along up-flow solid-phase denitrification column packed with

Shiyang Zhang1, Longqu Xiao1, Zhiwei Tang1

  • 1School of Civil Engineering and Architecture, Wuhan University of Technology, Wuhan 430070, PR China.

Bioresource Technology
|October 9, 2021
PubMed
Summary
This summary is machine-generated.

This study investigated solid-phase denitrification (SPD) using polycaprolactone (PCL) in up-flow columns. Denitrification primarily occurred at the bottom, achieving high nitrate removal, with minor nitrite and ammonium accumulation.

Keywords:
Filling areaMicrobial characterizationPolycaprolactoneSolid-phase denitrificationdissimilatory nitrate reduction to ammonium (DNRA)

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Area of Science:

  • Environmental Science
  • Microbiology
  • Chemical Engineering

Background:

  • Solid-phase denitrification (SPD) is crucial for wastewater treatment.
  • Polycaprolactone (PCL) is a potential material for packing SPD columns.
  • Understanding microbial and physicochemical profiles is key to optimizing SPD performance.

Purpose of the Study:

  • To investigate physicochemical and microbial characteristics in PCL-packed SPD columns at various filling heights.
  • To determine the contribution of different column sections to overall treatment performance.
  • To identify key microbial players and processes involved in denitrification.

Main Methods:

  • Up-flow solid-phase denitrification (SPD) columns packed with polycaprolactone (PCL).
  • Analysis of physicochemical parameters (e.g., nitrate, nitrite, ammonium) at different filling heights.
  • Microbial characterization to identify key genera and metabolic pathways.

Main Results:

  • Both PCL-filled and non-filled areas contributed significantly to treatment.
  • Denitrification predominantly occurred near the bottom of the filling column.
  • High nitrate removal (≥98.7%) was observed, with minimal nitrite and ammonium accumulation.
  • PCL hydrolysis was more intense in the top substrate layer.
  • Dissimilatory nitrate reduction to ammonium (DNRA) and microbial lysis contributed to ammonium accumulation.
  • Nitrate removal occurred via traditional denitrification and DNRA.
  • Unclassified genera like JGI_0000069-P22 and Gracilibacteria may play a role in denitrification.

Conclusions:

  • Polycaprolactone (PCL) is effective in solid-phase denitrification (SPD) columns.
  • Optimizing filling height and nitrogen loading rate (NLR) is important for efficient nitrate removal.
  • Microbial processes, including DNRA and specific bacterial genera, are critical for nitrogen removal in SPD systems.