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Updated: Aug 1, 2026

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
Published on: December 16, 2016
Dropping the microbead: Source and sink related microplastic distribution in the Black Sea and Caspian Sea basins
Anouk D'Hont1, Adriaan Gittenberger2, Rob S E W Leuven3
1GiMaRIS, Marine Research Inventory & Strategy Solutions, Rijksstraatweg 75, 2171 AK Sassenheim, the Netherlands; Department of Animal Ecology and Physiology, Institute for Water and Wetland Research, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, the Netherlands.
Abstract:
Microplastic pollution is a growing, yet poorly understood problem. Here, we assessed the relationship between microplastic concentration and distance to rivers, shorelines, cities, sediment grain size or water depth in sediments of the world's largest (semi-)enclosed aquatic basins. Microplastic was extracted from sediment using density separation, elutriation and hydrophobic adhesion. Fibers and transparent or white microplastic particles were the most abundant shape and color. The microplastic concentration in sediments of the Black Sea was about twice as high compared to that in the Caspian Sea. Fragment concentrations decreased with depth, while fiber concentrations were independent of depth. Overall, no relationship with distance to shores, rivers and cities or with grain size was observed. However, within some depth classes concentrations were related to the distance from rivers, shores and cities.
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