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Updated: Jul 14, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
A core genome multilocus sequence typing scheme for Mycoplasma hyorhinis.
Moritz Bünger1, Magdalena Posch2, Julia Wiesauer2
1University of Veterinary Medicine Vienna, Institute of Microbiology, Veterinärplatz 1, 1210, Vienna, Austria; University of Veterinary Medicine Vienna, University Clinic for Swine, Veterinärplatz 1, 1210, Vienna, Austria.
Core genome MLST (cgMLST) effectively differentiates Mycoplasma hyorhinis strains, revealing high diversity in swine. This method offers superior resolution for tracking swine respiratory disease outbreaks and understanding pathogen population structures.
Area of Science:
- Veterinary Microbiology
- Genomics
- Epidemiology
Background:
- Mycoplasma hyorhinis is a swine pathogen causing significant economic losses in pigs.
- Limited understanding of M. hyorhinis epidemiology hinders effective disease control strategies.
- This study addresses the need for robust molecular tools to investigate M. hyorhinis outbreaks.
Purpose of the Study:
- To develop and evaluate a core genome multilocus sequence typing (cgMLST) scheme for Mycoplasma hyorhinis.
- To assess the discriminatory power of cgMLST compared to conventional MLST and core genome SNP analysis.
- To determine the suitability of cgMLST for epidemiological investigations of M. hyorhinis.
Main Methods:
- Whole genome sequencing of 81 M. hyorhinis strains from Austria and Germany.
- Development of a 453-target gene cgMLST scheme using Ridom© SeqSphere+ software.
- Comparative analysis of cgMLST, conventional MLST, and core genome SNP (cgSNP) approaches.
Main Results:
- cgMLST revealed high genetic diversity among M. hyorhinis isolates, with no identical allele profiles found.
- cgMLST demonstrated superior discriminatory power (Simpson's ID = 0.995) compared to conventional MLST (0.952 and 0.985).
- cgSNP analysis showed high congruence with cgMLST but lacked resolution for closely related strains.
Conclusions:
- cgMLST is the most suitable method for detailed epidemiological investigations of M. hyorhinis.
- This approach provides high resolution for outbreak analysis and understanding population structure.
- cgMLST facilitates enhanced insights into the epidemiology of Mycoplasma hyorhinis infections in swine.
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