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"AMPHICEZINE": NEW APPROACHES TO FIGHTING CANCER PRELIMINARY THEORETICAL AND EXPERIMENTAL (IN VITRO) MESSAGE
1Georgia Israel Joint Clinic GIDMEDI; Georgian Technical University, Tbilisi, Georgia.
Abstract:
Objectives - strategic objective - creation of fundamentally new inhibitors of metastasing of malignant tumors due to the radical surgeries carried out on them. Specific tactical objective - revealing the possible cytolytic and citostatic effectiveness of the drug "Amphicezine" created by us on atypical fibroblastic cells. The effect of "Amphicezine" on viable D60 p4 line cell count was measured using live cell imaging as a measure of cell proliferation. The results of "Amphicezine" treatment of D60 p4 cell line didn't shown a cytolytic/cytotoxic effect for dermal atypical fibroblast cells. Control cells and cells added 2.5% and 1% "Amphicezine" continued to proliferate, while cells treated with 5% and 10% "Amphicezine" didn't change cell count. The cell growth curves indicate viable cell count increase for control and 2.5%, 1% "Amphicezine" treated cells. 93.5% inhibition on D60 p4 were shown by 10% "Amphicezine" and 79.8% inhibition were shown on 5% "Amphicezine" treated cells. Cells treated with 2.5% and 1% "Amphicezine" showed similar proliferation profile as the control sample. The effect of "Amphicezine" on viable D60 p4 cell line cell count was measured using live cell imaging as a measure of cell proliferation. Maximal inhibition on D60 p4 cells were shown by 10% "Amphicezine" (93.5%). The results show that the growth curves for viable D60 p4 cell culture didn't indicate a cytolytic / cytotoxic effect for all samples (concentrations) treated with "Amphicezine". Cells treated with 2.5% and 1% "Amphicezine" showed similar proliferation profile as the control (PBS) sample.
Insights
Amphicezine demonstrated significant anti-proliferative effects on atypical fibroblast cells, inhibiting D60 p4 cell growth by up to 93.5%. However, it did not exhibit a direct cytolytic or cytotoxic impact on these cells.
Area of Science:
- Oncology
- Cell Biology
- Drug Discovery
Background:
- Metastasis remains a critical challenge in malignant tumor treatment, often necessitating radical surgeries.
- Developing novel inhibitors to prevent tumor metastasis is a key strategic objective in cancer research.
Purpose of the Study:
- To evaluate the cytolytic and cytostatic effectiveness of Amphicezine on atypical fibroblastic cells.
- To assess the potential of Amphicezine as an anti-metastatic agent.
Main Methods:
- Utilized live cell imaging to measure cell proliferation and count in the D60 p4 cell line.
- Treated D60 p4 cells with varying concentrations of Amphicezine (1%, 2.5%, 5%, 10%) and a control (PBS).
- Analyzed cell growth curves to determine the impact of Amphicezine on cell viability and proliferation.
Main Results:
- Amphicezine did not display a direct cytolytic or cytotoxic effect on dermal atypical fibroblast cells.
- Cell proliferation was observed in control groups and those treated with 1% and 2.5% Amphicezine.
- Significant inhibition of D60 p4 cell proliferation was noted at higher concentrations: 79.8% with 5% Amphicezine and 93.5% with 10% Amphicezine.
Conclusions:
- Amphicezine exhibits potent cytostatic properties, effectively inhibiting the proliferation of atypical fibroblast cells at concentrations of 5% and 10%.
- While not directly cytolytic, Amphicezine's anti-proliferative action suggests potential in managing tumor metastasis.
- Further investigation is warranted to explore Amphicezine's therapeutic efficacy in preventing cancer metastasis.
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