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Updated: Oct 17, 2025

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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CE-MS for Proteomics and Intact Protein Analysis.

Valeriia O Kuzyk1, Govert W Somsen1, Rob Haselberg2

  • 1Division of Bioanalytical Chemistry, AIMMS: Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.

Advances in Experimental Medicine and Biology
|October 10, 2021
PubMed
Summary
This summary is machine-generated.

This chapter details capillary electrophoresis hyphenated to mass spectrometry (CE-MS) for protein and peptide analysis. It covers techniques from sample preparation to mass spectrometry, highlighting applications in PTMs, biomarker discovery, and biotechnology.

Keywords:
Biomarker discoveryBiopharmaceuticals analysisCE separation optimizationCE-MS interfacingCE-MS, ProteomicsIntact protein analysisMS fragmentationPTM analysisSample concentration techniques

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Proteomics

Background:

  • Capillary electrophoresis hyphenated to mass spectrometry (CE-MS) is a powerful analytical technique.
  • Analyzing proteins, peptides, and posttranslational modifications (PTMs) presents unique challenges.
  • Optimizing CE-MS parameters is crucial for high-end analytical performance.

Purpose of the Study:

  • To provide a comprehensive overview of parameters for high-end CE-MS analysis of proteins and peptides.
  • To detail the CE-MS system from sample handling to mass detection.
  • To present diverse applications of CE-MS in scientific research and industry.

Main Methods:

  • Exploration of sample preconcentration and injection techniques.
  • Discussion of capillary electrophoresis parameters.
  • Consideration of mass spectrometry instrumentation and settings.

Main Results:

  • A structured approach to understanding the CE-MS workflow.
  • Identification of key parameters influencing analytical outcomes.
  • Demonstration of CE-MS applicability in PTMs, biomarker discovery, and biotechnology.

Conclusions:

  • Optimized CE-MS enables detailed analysis of complex biological samples.
  • The presented framework aids researchers in method development and application.
  • CE-MS is a versatile tool with significant impact across various scientific disciplines.