Graphenoxide Cross-Linker Based Potentiometric Biosensor Design for Sarcosine Determination

Area of Science:

• Biomedical Engineering
• Biosensor Technology
• Cancer Diagnostics

Background:

• Sarcosine (N-methyl glycine) is an amino acid and a potential biomarker for prostate cancer.
• Elevated sarcosine levels in serum and urine indicate active prostate cancer malignancy.

Purpose of the Study:

• To design a highly selective and stable potentiometric biosensor for sarcosine detection.
• To achieve a low detection limit, fast response time, and wide linear range for sarcosine measurement.

Main Methods:

• Development of a Graphene oxide (GFOX) photosensitive cross-linker based potentiometric biosensor using the ANADOLUCA method.
• Characterization of GFOX using Raman, FT-IR, XPS, TEM, and AFM.
• Evaluation of the biosensor's performance for sarcosine determination.

Main Results:

• The GFOX biosensor detected 10⁻⁴ mM sarcosine within 60 seconds.
• Achieved a low detection limit of 9.45x10⁻⁷ mM and a linear range of 10⁻¹ to 10⁻⁵ mM.
• Demonstrated high selectivity for sarcosine over glycine and excellent reusability with no loss of sensitivity.

Conclusions:

• A novel GFOX-based potentiometric biosensor for sarcosine determination was successfully developed.
• The use of GFOX photosensitive cross-linkers enhanced biosensor stability and efficiency.
• The developed biosensor shows promise for the early diagnosis of prostate cancer.