Related Experiment Video

Updated: Oct 17, 2025

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
09:13

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Published on: July 13, 2017

9.6K

Profile of Immunoglobulin G N-Glycome in COVID-19 Patients: A Case-Control Study

Haifeng Hou1, Huan Yang2,3, Pengcheng Liu1

  • 1School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai'an, China.

Frontiers in Immunology
|October 11, 2021
PubMed

Insights

Changes in IgG glycosylation impact COVID-19 severity. This study found decreased IgG fucosylation and sialylation in patients, affecting immune responses and disease pathogenesis.

Area of Science:

  • Immunology
  • Glycobiology
  • Infectious Diseases

Background:

  • Coronavirus disease 2019 (COVID-19) poses a global health challenge.
  • Alterations in immunoglobulin G (IgG) glycosylation patterns are linked to COVID-19 severity.

Purpose of the Study:

  • To compare IgG N-glycome profiles between COVID-19 patients and healthy controls.
  • To investigate the association between IgG glycosylation changes and COVID-19 pathogenesis.

Main Methods:

  • A case-control study involving 104 COVID-19 patients and 104 healthy controls.
  • Serum IgG N-glycome composition analyzed using hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography (HILIC-UPLC).

Main Results:

  • COVID-19 patients exhibited decreased IgG fucosylation, potentially upregulating antibody-dependent cell cytotoxicity (ADCC).
  • Severe COVID-19 cases showed reduced IgG sialylation, contributing to ADCC-enhanced inflammatory cytokines.
  • Decreased IgG sialylation and galactosylation were implicated in COVID-19 pathogenesis via complement pathway activation.

Conclusions:

  • IgG N-glycosylation patterns are significantly altered in COVID-19 patients.
  • These glycosylation changes are associated with altered immune responses, including ADCC and complement activation.
  • IgG N-glycosylation plays a crucial role in the complex clinical manifestations of SARS-CoV-2 infection.

Keywords:
COVID-19IgGSARS-CoV-2case-control studyglycosylation

More Related Videos

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography11:01

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Published on: January 18, 2020

8.7K
Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-1908:48

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

3.0K

Related Concept Videos

Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
21.1K
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.5K
Transcytosis of IgG01:15

Transcytosis of IgG

Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
3.2K
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
6.1K