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Self-resetting molecular probes for nucleic acids detection enabled by fuel dissipative systems
1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Nano Today
|October 11, 2021
Summary
A novel self-resetting molecular probe detects SARS-CoV-2 RNA repeatedly using DNA nanotechnology. This innovation enhances infectious disease screening and global public health strategies.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Biology
Background:
- The COVID-19 pandemic caused significant global health and economic damage.
- Accurate and rapid detection of SARS-CoV-2 is crucial for disease control.
Purpose of the Study:
- To develop a self-resetting molecular probe for repeated SARS-CoV-2 RNA detection.
- To assess the probe's capability for identifying single-nucleotide variants and other viruses.
Main Methods:
- Utilized simulation toolkits for probe design and optimization.
- Employed DNA nanotechnology to orchestrate a fuel dissipative system.
- Investigated probe performance using cyclic detections and kinetic analysis.
Main Results:
- Achieved highly consistent signal amplitudes across cyclic detections.
- Demonstrated unique fingerprint signals for high-confidence single-nucleotide variant identification.
- Validated generic detection capability and superior orthogonality against ZIKV, MERS-CoV, and SARS-CoV.
- Correctly classified all clinical samples from COVID-19 patients and controls.
Conclusions:
- The self-resetting molecular probe offers enhanced screening for COVID-19 and other infectious diseases.
- This technology has the potential to improve disease control measures.
- The probe contributes to building a broader global public health agenda.
