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Updated: Oct 17, 2025

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
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circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D

Kai Ren1, Buying Li2, Liqing Jiang1

  • 1Department of Cardiovascular Surgery, Xijing Hospital, Air Force Military Medical University, Xi'an, China.

Oxidative Medicine and Cellular Longevity
|October 11, 2021
PubMed

Insights

Circular RNA circ_0023461 exacerbates acute myocardial infarction by impairing cardiomyocyte function. Silencing circ_0023461 protects against hypoxia-induced damage via the microRNA-370-3p/phosphodiesterase 4D pathway.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Acute myocardial infarction (AMI) is a leading cause of cardiovascular morbidity and mortality.
  • Circular RNAs (circRNAs) play a role in the pathogenesis of various diseases, including AMI.
  • The function of novel circRNAs, such as circ_0023461, in hypoxia-induced cardiomyocyte injury remains to be elucidated.

Purpose of the Study:

  • To investigate the role and mechanism of circ_0023461 in hypoxia-induced cardiomyocyte dysfunction.
  • To explore the potential therapeutic target of circ_0023461 in the context of AMI.

Main Methods:

  • Gene expression analysis using RT-qPCR and Western blot.
  • Assessment of cardiomyocyte viability, proliferation, migration, apoptosis, oxidative stress, inflammation, and glycolysis.
  • Investigation of molecular interactions using dual-luciferase reporter and RNA pull-down assays.

Main Results:

  • circ_0023461 expression is elevated in AMI patients and hypoxia-treated cardiomyocytes.
  • circ_0023461 knockdown mitigates hypoxia-induced cardiomyocyte injury, including improved viability, proliferation, migration, and glycolysis, and reduced apoptosis, oxidative stress, and inflammation.
  • circ_0023461 acts as a molecular sponge for microRNA-370-3p (miR-370-3p), upregulating phosphodiesterase 4D (PDE4D) expression.

Conclusions:

  • circ_0023461 knockdown exerts protective effects against hypoxia-induced cardiomyocyte dysfunction.
  • The protective mechanism involves the circ_0023461/miR-370-3p/PDE4D axis.
  • circ_0023461 represents a potential therapeutic target for AMI.
Abstract

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