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Hepatitis C virus subtyping in Uttarakhand, India: a comparative study.

Kuhu Chatterjee1, Deepjyoti Kalita1, Balram Ji Omar1

  • 1Department of Microbiology, All India Institute of Medical Sciences, Veerbhadra Marg, Dehradun, Rishikesh, 249203 Uttarakhand India.

Virusdisease
|October 11, 2021
PubMed
Summary

Reverse Hybridisation Assay (RHA) offers a faster, cheaper method for determining Hepatitis C Virus (HCV) genotype and subtypes compared to sequencing. While accurate, strict quality control is essential for reliable results in HCV genotyping.

Keywords:
Hepatitis C virus; Genotype; Subtype; Reverse hybridisation assay; Conventional sequencing

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Area of Science:

  • Virology
  • Molecular Diagnostics
  • Public Health

Background:

  • Hepatitis C Virus (HCV) infection remains a significant global health concern.
  • Accurate genotyping is crucial for effective treatment and epidemiological studies.

Purpose of the Study:

  • To compare the accuracy and efficiency of Reverse Hybridisation Assay (RHA) against conventional sequencing for HCV genotyping and subtyping.
  • To evaluate the diagnostic performance metrics of RHA in determining HCV genotype and subtype.

Main Methods:

  • HCV RNA was extracted from patient samples.
  • Viral load determination was performed.
  • Qualitative real-time PCR RHA and conventional sequencing were used for genotyping and subtyping.

Main Results:

  • RHA demonstrated high accuracy for genotype (96.55%) and subtype (89.66%) determination compared to sequencing.
  • The qualitative PCR showed sensitivity of 82.29% and specificity of 100%.

Conclusions:

  • RHA is a time-saving and cost-effective method for HCV genotype and subtype determination.
  • Results from RHA require careful interpretation, emphasizing the need for strict quality control measures.