Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Modulated electro-hyperthermia enhances tumor cell susceptibility to γδ T cell-mediated cytotoxicity and tumor infiltration in NOD/SCID xenograft models.

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·2026

Same author

Real-world costs per life-year of targeted therapy, incidence, lifetime health impact, and medical costs of renal cell carcinoma in Taiwan.

Frontiers in public health·2026

Same author

Dual Osteoprotective Actions of the Kefir Peptide KFP-1: Enhancement of Bone Formation and Suppression of Bone Resorption in Cells and Murine Models.

Calcified tissue international·2026

Same author

Single-Port Transoral Robotic Surgery for Head and Neck Lesions and Obstructive Sleep Apnea: A Prospective Real-World Case Series From Taiwan.

Laryngoscope investigative otolaryngology·2026

Same author

Missense and Intronic Variants in <i>HNF1A</i> Affect Prostate Cancer Aggressiveness in Patients with Biochemical Recurrence.

International journal of medical sciences·2026

Same author

Pubovesical Complex-Sparing Under Hypothermia During Robotic-Assisted Laparoscopic Radical Prostatectomy: A Single-Institution Case Series.

Journal of clinical medicine·2025

Search research articles

Related Experiment Video

Updated: Oct 17, 2025

Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats

Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats

Published on: March 30, 2018

The hernia sac-A suitable source for obtaining mesenchymal stem cells.

Alpha Dian-Yu Lin^1,2,3, Min-Che Tung², Chin-Heng Lu²

¹Joshua Taipei Hernia Center, Central Clinic & Hospital, Taipei, Taiwan.

Surgery Open Science

|October 11, 2021

Summary

Inguinal hernia sacs contain mesenchymal stem cells. These cells can be harvested from discarded tissue, offering a novel source for regenerative medicine applications.

Keywords:

hemia sac inguinal hemia mesenchymal stem cell

More Related Videos

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Published on: April 5, 2010

Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Published on: April 7, 2023

Related Experiment Videos

Last Updated: Oct 17, 2025

Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats

Evaluation of Stem Cell Therapies in a Bilateral Patellar Tendon Injury Model in Rats

Published on: March 30, 2018

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Published on: April 5, 2010

Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Published on: April 7, 2023

Area of Science:

Regenerative Medicine
Cell Biology
Surgical Innovation

Background:

Inguinal hernia sac tissue, a peritoneal extension, enlarges with disease progression.
Mesenchymal stem cells (MSCs) are hypothesized to play a role in hernia sac development.
Hernia sacs are typically discarded as medical waste after resection.

Purpose of the Study:

To investigate the presence of MSCs within inguinal hernia sac tissue.
To determine if hernia sacs can serve as a source for MSC harvesting.
To evaluate the potential of using resected hernia sacs for regenerative medicine.

Main Methods:

Resected hernia sacs from 4 patients were processed for cell extraction.
Mesenchymal stem cell presence was confirmed using specific cell surface markers (CD105, CD73, CD90) and lack of hematopoietic markers (CD45, CD34, etc.).
Cell differentiation potential was assessed via von Kossa, Alcian blue, and Oil Red O staining.

Main Results:

Cells isolated from hernia sacs exhibited typical MSC morphology and plastic adherence.
High expression of MSC markers (CD73, CD90, CD105) and low expression of hematopoietic markers confirmed MSC identity.
In vitro cell cultures demonstrated differentiation into osteoblasts, adipocytes, and chondroblasts.

Conclusions:

Inguinal hernia sacs represent a novel and accessible source of mesenchymal stem cells.
These MSCs possess differentiation potential, suitable for future therapeutic applications.
Discarded hernia sac tissue can be repurposed for regenerative medicine, reducing medical waste.