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Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
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A bilayered tissue engineered in vitro model simulating the tooth periodontium.

A Khadre, E Lm Raif, S Junaid

  • 1Department of Oral Biology, Leeds School of Dentistry, University of Leeds, UK.R.El-Gendy@leeds.ac.uk.

European Cells & Materials
|October 11, 2021
PubMed
Summary

Researchers developed a bilayered construct mimicking tooth attachment for tissue regeneration. This model supports human periodontal ligament cells (HPDLCs) and shows promise for studying tooth regeneration and loading effects.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Periodontology

Background:

  • Regenerating the tooth periodontium is challenging due to its complex structure.
  • A need exists for in vitro models simulating human tooth attachment for research and therapeutic development.

Purpose of the Study:

  • To create a bilayered in vitro construct simulating the periodontal ligament and alveolar bone.
  • To enable tissue regeneration and investigate physiological and orthodontic loading on tooth attachment.

Main Methods:

  • Fabrication of a bilayered construct using a sol-gel 60S10Mg scaffold (bone) and a collagen membrane (ligament).
  • Seeding scaffolds with human periodontal ligament cells (HPDLCs) and culturing them separately or in a bilayered construct for two weeks.
  • Conducting biological (cell viability, SEM, gene expression) and mechanical characterization of scaffolds and constructs.

Main Results:

  • Human periodontal ligament cells (HPDLCs) demonstrated good viability and attachment within the bilayered construct.
  • No decrease in cellular gene expression for periodontium regeneration markers was observed in the bilayered construct compared to individual scaffolds.
  • The construct provided a biocompatible 3D environment for HPDLCs.

Conclusions:

  • The developed bilayered construct effectively simulates key components of tooth attachment.
  • This model supports HPDLCs and maintains their regenerative gene expression, indicating its potential for tooth regeneration research.
  • The construct is suitable for investigating mechanical loading effects on periodontal tissues.