A Pyrazolo Imine-based Colorimetric and Turn-on Fluorescent Sensor Probe for Determination of Hg²⁺ Ion and its...

In this work, we synthesized diethylamino substituted pyrazolo imine (3) fluorescent probe for recognition of Hg²⁺ ion.The sensor probe 3 can detect Hg²⁺ by colorimetric method, and there is a 10-fold enhancement in fluorescence response. When the fluorescent probe bound with Hg²⁺ ion, turn-on fluorescence was observed via the coordination. Probe 3 has an excellent selectivity toward Hg²⁺ in the CH₃ CN/H₂ O (8:2, v/v) solution with low limit of detection and high binding association constant of 551 parts per billion (ppb) and 6.6067 × 10⁶  m^-1 for 3+Hg²⁺ , respectively. Furthermore, the formation of 3+Hg²⁺ complex with 1:1 binding mode was evidenced by Job's plot, ¹ H NMR spectroscopy and Mass analysis. In addition, probe 3 is a feasible option to detect Hg²⁺ in various sources of water samples. Bio-imaging experiments have demonstrated that probe 3 can be used to monitor Hg²⁺ in Escherichia coli bacterial cell. The sensor 3 was also used for paper strip application to detect Hg²⁺ ion.