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CircRNA CircZMYM4 inhibits the growth and metastasis of lung adenocarcinoma via the miR-587/ODAM pathway

Dong-Feng Yuan1, Hao-Ran Wang1, Zong-Fei Wang1

  • 1Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Biochemical and Biophysical Research Communications
|October 11, 2021
PubMed

Insights

Circular RNAs (circRNAs) regulate lung adenocarcinoma (LUAD) by down-regulating circZMYM4. This study shows circZMYM4 suppresses LUAD progression by targeting the miR-587/ODAM axis, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in tumorigenesis.
  • Lung adenocarcinoma (LUAD) is a significant health concern with complex regulatory mechanisms.
  • Understanding novel molecular players in LUAD is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the role of circZMYM4 in LUAD progression.
  • To elucidate the molecular mechanism underlying circZMYM4's function in LUAD.
  • To evaluate circZMYM4 as a potential biomarker and therapeutic target for LUAD.

Main Methods:

  • CircRNA microarray analysis of LUAD tissues.
  • RT-PCR and bioinformatics analysis (GEO, TCGA datasets) to assess circZMYM4 expression.
  • In vivo and in vitro gain- and loss-of-function experiments.
  • Dual-luciferase reporter assays and RNA-pull down to confirm molecular interactions.

Main Results:

  • CircZMYM4 expression was significantly downregulated in LUAD tissues and cell lines.
  • Overexpression of circZMYM4 inhibited LUAD cell proliferation, metastasis, and apoptosis.
  • CircZMYM4 acts as a sponge for miR-587, upregulating ODAM and thereby suppressing LUAD progression.
  • ODAM knockdown reversed the inhibitory effects of circZMYM4 overexpression.

Conclusions:

  • CircZMYM4 functions as a tumor suppressor in LUAD by regulating the miR-587/ODAM axis.
  • CircZMYM4 holds promise as a potential diagnostic biomarker and therapeutic target for LUAD.
Keywords:
CircZMYM4Lung adenocarcinomaMetastasisODAMceRNAmiR-587

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