Evaluate the bisphenol A-induced redox state in cells, zebrafish and in vivo with a hydrogen peroxide turn-on...

Hydrogen peroxide (H₂O₂) is an important active oxygen species that plays a major role in redox balance and in physiological and pathological processes of various diseases of biological systems. As H₂O₂ is an endogenous active molecule, fluctuations in H₂O₂ content are not only affected by the state of biological system itself but also easily affected by Bisphenol A (BPA, a typical estrogenic environmental pollutant) in the external environment. Here, the near-infrared fluorescent probe Cy-NOH2 (λ_em = 750 nm) as a tool was synthesized to detect fluctuations in H₂O₂ content in cells and organisms induced by BPA. High sensitivity and excellent selectivity were found when the probe Cy-NOH2 was used to monitor endogenous H₂O₂ in vitro. In addition, the expression of H₂O₂ induced by different concentrations of BPA was able to be detected by the probe. Zebrafish and mice models were induced with different concentrations of BPA, and the H₂O₂ content showed significant increasing trends in zebrafish and livers of mice with increasing BPA concentrations. This study reveals that the probe Cy-NOH2 can be used as an effective tool to monitor the redox state in vivo under the influence of BPA, which provides a basis for clarifying the mechanisms of BPA in a variety of physiological and pathological processes.