Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

8.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A platelet-monocyte pyroptotic axis mediates uremic cardiomyopathy: critical role of PF4 and hematopoietic caspase-1.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2026
Same author

Stage-associated remodeling of CD169 macrophage states is linked to immune regulation in colitis-associated colorectal cancer.

Journal of gastroenterology·2026
Same author

Immunological research landscapes and emerging immune mechanisms in HIV/HBV co-infection: a bibliometric analysis (2014-2024).

Frontiers in immunology·2026
Same author

Electrophysiological and Molecular Features of Remdesivir-Induced Cardiac Toxicity in Male and Female Guinea Pigs.

International journal of molecular sciences·2026
Same author

Light Chain Deposition Disease With Trace Monoclonal Proteinemia as Severe Renal Failure: A Case Image.

Clinical case reports·2026
Same author

Giant electric field dependent hole mobility of CsPbBr<sub>3</sub> nanocrystal films.

Nanoscale·2026

Related Experiment Video

Updated: Oct 17, 2025

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

4.7K

Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout.

Guangqi Chen1, Jiafen Cheng1, Hanjie Yu1

  • 1Center for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, PR China.

Proteome Science
|October 12, 2021
PubMed
Summary

Plasma protein profiles can distinguish between acute gout, remission, and asymptomatic hyperuricemia. Histone H2A, Histone H2B, and Thrombospondin-1 (THBS1) show potential as biomarkers for gout progression and management.

Keywords:
GoutHistone H2AHistone H2BPRMTHBS1iTRAQ

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.2K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

5.6K

Related Experiment Videos

Last Updated: Oct 17, 2025

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

4.7K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.2K
Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

5.6K

Area of Science:

  • Biochemistry
  • Immunology
  • Proteomics

Background:

  • Gout is a complex inflammatory arthritis linked to hyperuricemia.
  • Distinguishing asymptomatic hyperuricemia (AHU) from acute gout (AG) or remission of gout (RG) is challenging.
  • Predicting gout flares and remission progression requires better diagnostic tools.

Purpose of the Study:

  • To identify plasma protein profiles differentiating between AG, RG, AHU patients, and healthy controls.
  • To explore potential protein biomarkers for gout status.
  • To gain insights into the molecular mechanisms underlying gout progression.

Main Methods:

  • Utilized isobaric tags for relative and absolute quantification (iTRAQ) and parallel reaction monitoring (PRM) for plasma protein analysis.
  • Analyzed plasma samples from AG (n=8), RG (n=7), AHU (n=7), and healthy control (n=8) groups.
  • Quantified and compared protein expression levels across different patient cohorts.

Main Results:

  • Identified eleven differentially expressed proteins, including Histone H2A, Histone H2B, Thrombospondin-1 (THBS1), Myeloperoxidase (MPO), and complement components.
  • Histone H2A, Histone H2B, and THBS1 emerged as key regulators in gout progression.
  • Complement and coagulation cascades were identified as significant functional pathways in gout pathogenesis.

Conclusions:

  • Histone H2A, Histone H2B, and THBS1 are potential biomarkers for distinguishing gout attack from AHU or RG.
  • These proteins offer novel theoretical insights for gout prognosis, treatment, and management.
  • The findings contribute to a better understanding of gout's underlying molecular mechanisms.