Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comment on: Continuous versus intermittent anticoagulation during endoscopic treatment of esophageal varices in cirrhosis: a randomized controlled trial.

Therapeutic advances in gastroenterology·2026
Same author

3D Cell Printing and Manipulation with Magnetic Bioinks.

Biomedicines·2026
Same author

Ultrasound priming gated by solid tumor hallmarks to guide CAR-T therapy.

Science advances·2026
Same author

Evaluation of the National Viral Hepatitis Control Program in Uttarakhand: A mixed-method approach.

Journal of family medicine and primary care·2026
Same author

When the lung is at risk: diagnosis, manifestations, and complications of pulmonary sarcoidosis.

Current opinion in immunology·2026
Same author

Epidemiology, Predictors of Antimicrobial Resistance and Empiric Treatment Strategies for Spontaneous Bacterial Peritonitis in Cirrhosis: A Multicentre SBP-INDIA Study.

Alimentary pharmacology & therapeutics·2026

Related Experiment Video

Updated: Oct 17, 2025

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.4K

SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles.

Srivatsa Aithal1, Sarah Mishriki2, Rohit Gupta1

  • 1Department of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada.

Talanta
|October 12, 2021
PubMed
Summary
This summary is machine-generated.

A new SARS-CoV-2 detection test uses functionalized gold nanoprobes to identify the virus. This rapid diagnostic method offers potential for effective monitoring of virus spread and containment strategies.

Keywords:
AptamerBiosensingGold nanoparticleSARS-CoV-2Spike proteinSurface plasmon resonance

More Related Videos

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

1.8K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.4K

Related Experiment Videos

Last Updated: Oct 17, 2025

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
08:23

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

12.4K
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
12:23

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

1.8K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.4K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Virology

Background:

  • The urgent need for rapid diagnostics to monitor SARS-CoV-2 spread and containment.
  • Limitations of current detection methods for timely and widespread application.

Purpose of the Study:

  • To develop and validate a rapid detection test for SARS-CoV-2 using aptamer-functionalized gold nanoprobes.
  • To assess the efficacy of the nanoprobe assay in detecting SARS-CoV-2 spike protein and viral particles.

Main Methods:

  • Functionalization of gold nanoparticles with SARS-CoV-2 spike protein-specific aptamers.
  • Utilizing a coagulant-induced agglomeration assay based on changes in plasmon absorbance spectra.
  • Detection of spike protein concentration via time-varying light absorbance at 540 nm.
  • Quantification of inactivated SARS-CoV-2 using the developed nanoprobe assay.

Main Results:

  • Confirmation of aptamer binding to the SARS-CoV-2 spike protein using enzyme-linked immunosorbent assay.
  • Detection of 16 nM and higher concentrations of spike protein in phosphate-buffered saline.
  • Correlation between protein concentration and critical coagulant concentration for nanoprobe agglomeration.
  • Successful detection of 3540 genome copies/μl of inactivated SARS-CoV-2.

Conclusions:

  • The developed nanoprobe assay provides a rapid and sensitive method for SARS-CoV-2 detection.
  • This aptamer-based gold nanoparticle approach shows promise for point-of-care diagnostics and epidemiological surveillance.
  • The assay's ability to detect viral particles and specific proteins facilitates effective virus monitoring.