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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
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A molecularly imprinted biosensor based on water-compatible and electroactive polymeric nanoparticles for lysozyme
Xiaoliang Yang1, Huan Liu1, Yuting Ji1
1Anhui Laboratory of Clean Energy Materials and Chemistry for Sustainable Conversion of Natural Resources, School of Chemical and Environmental Engineering, Anhui Polytechnic University, Wuhu, 241000, China.
Talanta
|October 12, 2021
Summary
This study presents a novel biosensor for detecting lysozyme (LYS) using molecular imprinting. The developed biosensor demonstrates high sensitivity and selectivity for accurate lysozyme quantification.
Area of Science:
- Biomolecular Engineering
- Biosensor Technology
- Electrochemistry
Background:
- Lysozyme (LYS) is a crucial enzyme in biological systems.
- Developing selective and sensitive detection methods for LYS is essential for diagnostics and research.
- Existing biosensors often face challenges with stability and sensitivity in aqueous environments.
Purpose of the Study:
- To develop a molecularly imprinted biosensor for sensitive and selective lysozyme detection.
- To utilize water-soluble and electroactive polymers for enhanced biosensor performance.
- To investigate the impact of electro-polymerization on recognition site stability and conductivity.
Main Methods:
- Preparation of polymer nanoparticles from water-soluble poly(γ-glutamic acid) modified with 3-aminothiophene.
- Molecular imprinting of lysozyme in aqueous solution.
- Electro-polymerization to create a conductive polymer network and lock recognition sites.
- Electrochemical characterization and performance evaluation of the biosensor.
Main Results:
- The biosensor demonstrated a wide linear detection range for lysozyme from 1 × 10⁻¹⁰ to 1 × 10⁻⁵ mg mL⁻¹.
- Achieved satisfactory selectivity, stability, and repeatability in lysozyme detection.
- Electro-polymerization enhanced electron transfer rate and film conductivity.
Conclusions:
- The developed molecularly imprinted biosensor offers a promising platform for accurate lysozyme quantification.
- The use of water-soluble electroactive polymers facilitates effective imprinting and improved biosensor performance.
- The biosensor exhibits excellent analytical characteristics for potential applications in diagnostics.
