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Updated: Oct 17, 2025
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
A molecularly imprinted biosensor based on water-compatible and electroactive polymeric nanoparticles for lysozyme
Xiaoliang Yang1, Huan Liu1, Yuting Ji1
1Anhui Laboratory of Clean Energy Materials and Chemistry for Sustainable Conversion of Natural Resources, School of Chemical and Environmental Engineering, Anhui Polytechnic University, Wuhu, 241000, China.
Abstract:
A molecularly imprinted biosensor for lysozyme based on the polymer nanoparticles self-assembled from water-soluble and electroactive poly (γ-glutamic acid) modified with 3-aminothiophene copolymer were prepared. The water-soluble copolymer made imprinting of lysozyme in aqueous solution possible and thus facilitated improvement of the activity of LYS. Subsequent electro-polymerization not only locked the recognition site between copolymer and lysozyme but also created a conductive polymer network, which can enhance the electron transfer rate and increase the conductivity of the film. The prepared molecularly imprinted biosensor exhibited a wide linear range from 1 × 10-10 to 1 × 10-5 mg mL-1, and satisfactory selectivity, stability, repeatability for lysozyme detection.
