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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.0K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

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NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
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2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

336
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
336
NMR and Mass Spectroscopy of Carboxylic Acids01:30

NMR and Mass Spectroscopy of Carboxylic Acids

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In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the...
4.4K
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

5.3K
Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
5.3K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

374
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
374

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Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
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The robust NMR toolbox for metabolomics.

Kousik Chandra1, Samah Al-Harthi1, Fatimah Almulhim1

  • 1Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia. Mariusz.jaremko@kaust.edu.sa.

Molecular Omics
|October 12, 2021
PubMed
Summary
This summary is machine-generated.

We developed new NMR experiments to identify metabolites in complex biological samples. These methods effectively remove background noise, improving metabolite detection in metabolomics research.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy

Background:

  • Metabolomics research often faces challenges in identifying metabolites due to overlapping signals from macromolecules like lipids and proteins.
  • Existing Nuclear Magnetic Resonance (NMR) techniques may struggle with sensitivity and specificity in complex biological matrices.

Purpose of the Study:

  • To implement and validate a set of selective and non-selective Carr-Purcell-Meiboom-Gill (CPMG) filtered 1D and 2D experiments.
  • To enhance the unambiguous identification of metabolites within complex biological samples.
  • To improve the performance of non-targeted metabolomics analysis.

Main Methods:

  • Utilized Carr-Purcell-Meiboom-Gill (CPMG) filtering in combination with 1D and 2D TOCSY (Total Correlation Spectroscopy) and HSQC (Heteronuclear Single Quantum Coherence) NMR experiments.
  • Applied selective and non-selective filtering strategies.
  • Validated the experimental suite for metabolomics applications.

Main Results:

  • The developed NMR experiments successfully facilitated the unambiguous identification of metabolites.
  • The methods effectively resolved metabolite signals from broad lipid and protein signals.
  • 2D spectra significantly improved non-targeted analysis by suppressing background signals from macromolecules.

Conclusions:

  • The suite of CPMG-filtered TOCSY/HSQC experiments provides a robust tool for metabolomics.
  • These advanced NMR techniques enhance metabolite identification accuracy and sensitivity in complex biological samples.
  • The improved spectral quality aids in comprehensive metabolic profiling.